Part:BBa_K2721023
SpyTag with GFP
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 689
Usage and Biology
The covalent nature of Tag-Catcher interaction has facilitated the processing of proteins into various materials forms including all-protein-based, chemically cross-linked hydrogels, functional layer-by-layer thin films, hybrid colloidal assemblies, and living materials[1]. The SpyTag/SpyCatcher system is a convenient protein coupling tool for irreversible peptide-protein ligation. According to papers, Spytag forms a covalent isopeptide bound to its protein partner SpyCatcher derived from Streptococcus pyogenes fibronectin-binding protein[2].
Fig1 Structure of SpyTag/SpyCatcher.[2]
Considering that the tag-catcher pairs are ideal for binding and immobilization, our team use them as scaffold of protein assembly. Here GFP is a reporter.
Approach and Result
The part was assembled with T7 promoter, RBS and T7 terminator in pET26b+ plasmid vector. E. coli strain BL21(DE3). After growing to an optical density of 0.6~0.8 at 600 nm, the protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) at 25℃. We get the protein product via sonication. With N-terminal His-tag, it can be purified by Ni-beads[3].
Fig2 Agarose Gel Electrophoresis. | M is marker. Lane 1-2: 2 replicates of SpyTag with GFP
Reference
1. J Fang, WB Zhang. Genetically Encoded Peptide-protein Reactive Pairs. Acta Polymerica Sinica 2018 Jan (4):429-444.
2. Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7.
3. P Hengen. Purification of His-Tag fusion proteins from Escherichia coli. Trends in Biochemical Sciences, 1995,20(7): 285-286.
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